DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)

Sampling event
最新版本 published by Leibniz Institute for the Analysis of Biodiversity Change (LIB) on 2月 2, 2026 Leibniz Institute for the Analysis of Biodiversity Change (LIB)
發布日期:
2026年2月2日
授權條款:
CC-BY 4.0

下載最新版本的 Darwin Core Archive (DwC-A) 資源,或資源詮釋資料的 EML 或 RTF 文字檔。

DwC-A資料集 下載 141 紀錄 在 English 中 (3 MB) - 更新頻率: 有可能更新,但不確知何時
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說明

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'. The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

This data and the data in the datasets "https://doi.org/10.15468/9pzhm6" and "https://doi.org/10.15468/9pzhm6" belongs to one study.

資料紀錄

此資源sampling event的資料已發佈為達爾文核心集檔案(DwC-A),其以一或多組資料表構成分享生物多樣性資料的標準格式。 核心資料表包含 141 筆紀錄。

亦存在 2 筆延伸集的資料表。延伸集中的紀錄補充核心集中紀錄的額外資訊。 每個延伸集資料表中資料筆數顯示如下。

Event (核心)
141
dnaDerivedData 
35766
Occurrence 
35766

此 IPT 存放資料以提供資料儲存庫服務。資料與資源的詮釋資料可由「下載」單元下載。「版本」表格列出此資源的其它公開版本,以便利追蹤其隨時間的變更。

版本

以下的表格只顯示可公開存取資源的已發布版本。

權利

研究者應尊重以下權利聲明。:

此資料的發布者及權利單位為 Leibniz Institute for the Analysis of Biodiversity Change (LIB)。 This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF 註冊

此資源已向GBIF註冊,並指定以下之GBIF UUID: 3fe20fcc-d684-44e6-823a-9121d06be1ac。  Leibniz Institute for the Analysis of Biodiversity Change (LIB) 發佈此資源,並經由GBIF Germany同意向GBIF註冊成為資料發佈者。

關鍵字

Samplingevent; Specimen

外部資料

此資源尚有其他格式可用

ASV-Registry https://doi.org/10.20363/6wdc-yh16 UTF-8 Microsoft Excel

聯絡資訊

Lara Hoffmann
  • 出處
  • 連絡人
Author, Content Contact, Data Owner
University of Duisburg-Essen
Duisburg
DE
Stefan Stoll
  • 出處
PI, Data Owner
University of Applied Sciences Trier
Trier
DE
Dominik Buchner
  • 出處
Author, Data Owner
University of Duisburg-Essen
DE
Birgit Rach
  • 使用者
Data Curator
Leibniz institute for the Analysis of Biodiversity Change - LIB
Bonn
DE
Birgit Rach
  • 使用者
Data curator
LIB - Leibniz Institute for the Analysis of Biodiversity Change
DE
University of Duisburg-Essen
Owner institute
DE
University of Applied Sciences Trier
Owner institute
DE

地理涵蓋範圍

Near the town Reipoltskirchen, Rhineland-Palatinate, Germany

界定座標範圍 緯度南界 經度西界 [-90, -180], 緯度北界 經度東界 [90, 180]

分類群涵蓋範圍

無相關描述

Kingdom Animalia
Order Symphypleona, Heteronemertea, Mermithida, Hemiptera, Sarcoptiformes, Opiliones, Philodinida, Sordariales, Pucciniales, Glomerellalles, Tubulaniformes, Polydesmida, Monostilifera, Enchytraeida, Adinetida, Onygenales, Siphonaptera, Ceramiales, Julida, Plectida, Raphidioptera, Blattodea, Microascales, Ixodida, Crassiclitellata, Glomerida, Thysanoptera, Neuroptera, Dermaptera, Parachaela, Anthoathecata, Erysiphales, Ephemeroptera, Araneae, Helotiales, Mucorales, Agaricales, Mesostigmata, Plecoptera, Chordeumatida, Triganglionata, Lepidoptera, Ostropales, Pleosporales, Diptera, Teloschistales, Entomobryomorpha, Glomerellales, Trichoptera, Arthoniales, Geophilomorpha, Polyporales, Neelipleona, Anura, Sporidiobolales, Lithobiomorpha, Eurotiales, Mycocaliciales, Orthoptera, Rhabditida, Lecanorales, Russulales, Hypocreales, Coleoptera, Chaetothyriales, Peltigerales, Mecoptera, Hymenoptera, Thelephorales, Boletales, Peronosporales, Psocodea, Cantharellales, Trombidiformes, Clupeiformes, Stylommatophora, Cystobasidiales, Poduromorpha, Dothideales, Isopoda

時間涵蓋範圍

起始日期 / 結束日期 2022-05-10 / 2022-08-26

計畫資料

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

計畫名稱 DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)
辨識碼 P142690-DNA-MB_2025
經費來源 Heinrich Böll foundation (P142690) and Trier University of Applied Sciences: Junior Researcher Fund

取樣方法

The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

研究範圍 The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

方法步驟描述:

  1. The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

引用文獻

  1. Hoffmann L, Stoll S, Rach B (2026). Butterfly abundance data of four different short rotation coppice (SRC) life stages. Version 1.2. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/9pzhm6 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/9pzhm6
  2. Hoffmann L, Stoll S, Huber J, Rach B (2026). Vegetation data of four different short rotation coppice (SRC) life stages. Version 1.6. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/phmw92 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/phmw92

額外的詮釋資料

替代的識別碼 3fe20fcc-d684-44e6-823a-9121d06be1ac
https://biocase.zfmk.de/ipt/resource?r=dna_metabarcoding_data_of_arthropods_src