DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)

Evento de amostragem
Versão mais recente published by Leibniz Institute for the Analysis of Biodiversity Change (LIB) on fev. 2, 2026 Leibniz Institute for the Analysis of Biodiversity Change (LIB)
Publication date:
2 de fevereiro de 2026
Licença:
CC-BY 4.0

Baixe a última versão do recurso de dados, como um Darwin Core Archive (DwC-A) ou recurso de metadados, como EML ou RTF:

Dados como um arquivo DwC-A download 141 registros em English (3 MB) - Frequência de atualização: desconhecido
Metadados como um arquivo EML download em English (41 KB)
Metadados como um arquivo RTF download em English (15 KB)

Descrição

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'. The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

This data and the data in the datasets "https://doi.org/10.15468/9pzhm6" and "https://doi.org/10.15468/9pzhm6" belongs to one study.

Registros de Dados

Os dados deste recurso de evento de amostragem foram publicados como um Darwin Core Archive (DwC-A), que é o formato padronizado para compartilhamento de dados de biodiversidade como um conjunto de uma ou mais tabelas de dados. A tabela de dados do núcleo contém 141 registros.

Também existem 2 tabelas de dados de extensão. Um registro de extensão fornece informações adicionais sobre um registro do núcleo. O número de registros em cada tabela de dados de extensão é ilustrado abaixo.

Event (core)
141
dnaDerivedData 
35766
Occurrence 
35766

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versões

A tabela abaixo mostra apenas versões de recursos que são publicamente acessíveis.

Direitos

Pesquisadores devem respeitar a seguinte declaração de direitos:

O editor e o detentor dos direitos deste trabalho é Leibniz Institute for the Analysis of Biodiversity Change (LIB). This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

Este recurso foi registrado no GBIF e atribuído ao seguinte GBIF UUID: 3fe20fcc-d684-44e6-823a-9121d06be1ac.  Leibniz Institute for the Analysis of Biodiversity Change (LIB) publica este recurso, e está registrado no GBIF como um publicador de dados aprovado por GBIF Germany.

Palavras-chave

Samplingevent; Specimen

Dados externos

Os dados de recurso também estão disponíveis em outros formatos

ASV-Registry https://doi.org/10.20363/6wdc-yh16 UTF-8 Microsoft Excel

Contatos

Lara Hoffmann
  • Originador ●
  • Ponto De Contato
Author, Content Contact, Data Owner
University of Duisburg-Essen
Duisburg
DE
Stefan Stoll
  • Originador
PI, Data Owner
University of Applied Sciences Trier
Trier
DE
Dominik Buchner
  • Originador
Author, Data Owner
University of Duisburg-Essen
DE
Birgit Rach
  • Usuário
Data Curator
Leibniz institute for the Analysis of Biodiversity Change - LIB
Bonn
DE
Birgit Rach
  • Usuário
Data curator
LIB - Leibniz Institute for the Analysis of Biodiversity Change
DE
University of Duisburg-Essen
Owner institute
DE
University of Applied Sciences Trier
Owner institute
DE

Cobertura Geográfica

Near the town Reipoltskirchen, Rhineland-Palatinate, Germany

Coordenadas delimitadoras Sul Oeste [-90, -180], Norte Leste [90, 180]

Cobertura Taxonômica

Nenhuma descrição disponível

Reino Animalia
Ordem Symphypleona, Heteronemertea, Mermithida, Hemiptera, Sarcoptiformes, Opiliones, Philodinida, Sordariales, Pucciniales, Glomerellalles, Tubulaniformes, Polydesmida, Monostilifera, Enchytraeida, Adinetida, Onygenales, Siphonaptera, Ceramiales, Julida, Plectida, Raphidioptera, Blattodea, Microascales, Ixodida, Crassiclitellata, Glomerida, Thysanoptera, Neuroptera, Dermaptera, Parachaela, Anthoathecata, Erysiphales, Ephemeroptera, Araneae, Helotiales, Mucorales, Agaricales, Mesostigmata, Plecoptera, Chordeumatida, Triganglionata, Lepidoptera, Ostropales, Pleosporales, Diptera, Teloschistales, Entomobryomorpha, Glomerellales, Trichoptera, Arthoniales, Geophilomorpha, Polyporales, Neelipleona, Anura, Sporidiobolales, Lithobiomorpha, Eurotiales, Mycocaliciales, Orthoptera, Rhabditida, Lecanorales, Russulales, Hypocreales, Coleoptera, Chaetothyriales, Peltigerales, Mecoptera, Hymenoptera, Thelephorales, Boletales, Peronosporales, Psocodea, Cantharellales, Trombidiformes, Clupeiformes, Stylommatophora, Cystobasidiales, Poduromorpha, Dothideales, Isopoda

Cobertura Temporal

Data Inicial / Data final 2022-05-10 / 2022-08-26

Dados Sobre o Projeto

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

Título DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)
Identificador P142690-DNA-MB_2025
Financiamento Heinrich Böll foundation (P142690) and Trier University of Applied Sciences: Junior Researcher Fund

Métodos de Amostragem

The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

Área de Estudo The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

Descrição dos passos do método:

  1. The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

Citações bibliográficas

  1. Hoffmann L, Stoll S, Rach B (2026). Butterfly abundance data of four different short rotation coppice (SRC) life stages. Version 1.2. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/9pzhm6 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/9pzhm6
  2. Hoffmann L, Stoll S, Huber J, Rach B (2026). Vegetation data of four different short rotation coppice (SRC) life stages. Version 1.6. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/phmw92 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/phmw92

Metadados Adicionais

Identificadores alternativos 3fe20fcc-d684-44e6-823a-9121d06be1ac
https://biocase.zfmk.de/ipt/resource?r=dna_metabarcoding_data_of_arthropods_src