DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)

Sampling event
Последняя версия опубликовано Leibniz Institute for the Analysis of Biodiversity Change (LIB) февр. 2, 2026 Leibniz Institute for the Analysis of Biodiversity Change (LIB)
Дата публикации:
2 февраля 2026 г.
Лицензия:
CC-BY 4.0

Скачайте последнюю версию данных этого ресурса в формате Darwin Core Archive (DwC-A) или метаданных ресурса в форматах EML или RTF:

Данные в формате DwC-A Скачать 141 Записи в English (3 MB) - Частота обновления: неизвестно
Метаданные в формате EML Скачать в English (41 KB)
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Описание

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'. The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

This data and the data in the datasets "https://doi.org/10.15468/9pzhm6" and "https://doi.org/10.15468/9pzhm6" belongs to one study.

Записи данных

Данные этого sampling event ресурса были опубликованы в виде Darwin Core Archive (DwC-A), который является стандартным форматом для обмена данными о биоразнообразии в виде набора из одной или нескольких таблиц. Основная таблица данных содержит 141 записей.

Также в наличии 2 таблиц с данными расширений. Записи расширений содержат дополнительную информацию об основной записи. Число записей в каждой таблице данных расширения показано ниже.

Event (core)
141
dnaDerivedData 
35766
Occurrence 
35766

Данный экземпляр IPT архивирует данные и таким образом служит хранилищем данных. Данные и метаданные ресурсов доступны для скачивания в разделе Загрузки. В таблице версий перечислены другие версии ресурса, которые были доступны публично, что позволяет отслеживать изменения, внесенные в ресурс с течением времени.

Версии

В таблице ниже указаны только опубликованные версии ресурса, которые доступны для свободного скачивания.

Права

Исследователи должны соблюдать следующие права:

Публикующей организацией и владельцем прав на данную работу является Leibniz Institute for the Analysis of Biodiversity Change (LIB). Эта работа находится под лицензией Creative Commons Attribution (CC-BY 4.0).

Регистрация в GBIF

Этот ресурс был зарегистрирован в GBIF, ему был присвоен следующий UUID: 3fe20fcc-d684-44e6-823a-9121d06be1ac.  Leibniz Institute for the Analysis of Biodiversity Change (LIB) отвечает за публикацию этого ресурса, и зарегистрирован в GBIF как издатель данных при оподдержке GBIF Germany.

Ключевые слова

Samplingevent; Specimen

Внешние данные

Ресурс также доступен в других форматах

ASV-Registry https://doi.org/10.20363/6wdc-yh16 UTF-8 Microsoft Excel

Контакты

Lara Hoffmann
  • Originator ●
  • Point Of Contact
Author, Content Contact, Data Owner
University of Duisburg-Essen
Duisburg
DE
Stefan Stoll
  • Originator
PI, Data Owner
University of Applied Sciences Trier
Trier
DE
Dominik Buchner
  • Originator
Author, Data Owner
University of Duisburg-Essen
DE
Birgit Rach
  • User
Data Curator
Leibniz institute for the Analysis of Biodiversity Change - LIB
Bonn
DE
Birgit Rach
  • User
Data curator
LIB - Leibniz Institute for the Analysis of Biodiversity Change
DE
University of Duisburg-Essen
Owner institute
DE
University of Applied Sciences Trier
Owner institute
DE

Географический охват

Near the town Reipoltskirchen, Rhineland-Palatinate, Germany

Ограничивающие координаты Юг Запад [-90, -180], Север Восток [90, 180]

Таксономический охват

Описание отсутсвует

Kingdom Animalia
Order Symphypleona, Heteronemertea, Mermithida, Hemiptera, Sarcoptiformes, Opiliones, Philodinida, Sordariales, Pucciniales, Glomerellalles, Tubulaniformes, Polydesmida, Monostilifera, Enchytraeida, Adinetida, Onygenales, Siphonaptera, Ceramiales, Julida, Plectida, Raphidioptera, Blattodea, Microascales, Ixodida, Crassiclitellata, Glomerida, Thysanoptera, Neuroptera, Dermaptera, Parachaela, Anthoathecata, Erysiphales, Ephemeroptera, Araneae, Helotiales, Mucorales, Agaricales, Mesostigmata, Plecoptera, Chordeumatida, Triganglionata, Lepidoptera, Ostropales, Pleosporales, Diptera, Teloschistales, Entomobryomorpha, Glomerellales, Trichoptera, Arthoniales, Geophilomorpha, Polyporales, Neelipleona, Anura, Sporidiobolales, Lithobiomorpha, Eurotiales, Mycocaliciales, Orthoptera, Rhabditida, Lecanorales, Russulales, Hypocreales, Coleoptera, Chaetothyriales, Peltigerales, Mecoptera, Hymenoptera, Thelephorales, Boletales, Peronosporales, Psocodea, Cantharellales, Trombidiformes, Clupeiformes, Stylommatophora, Cystobasidiales, Poduromorpha, Dothideales, Isopoda

Временной охват

Дата начала / Дата окончания 2022-05-10 / 2022-08-26

Данные проекта

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

Название DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)
Идентификатор P142690-DNA-MB_2025
Финансирование Heinrich Böll foundation (P142690) and Trier University of Applied Sciences: Junior Researcher Fund

Методы сбора

The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

Охват исследования The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

Описание этапа методики:

  1. The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

Библиографические ссылки

  1. Hoffmann L, Stoll S, Rach B (2026). Butterfly abundance data of four different short rotation coppice (SRC) life stages. Version 1.2. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/9pzhm6 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/9pzhm6
  2. Hoffmann L, Stoll S, Huber J, Rach B (2026). Vegetation data of four different short rotation coppice (SRC) life stages. Version 1.6. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/phmw92 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/phmw92

Дополнительные метаданные

Альтернативные идентификаторы 3fe20fcc-d684-44e6-823a-9121d06be1ac
https://biocase.zfmk.de/ipt/resource?r=dna_metabarcoding_data_of_arthropods_src