Description
The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'. The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.
The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.
This data and the data in the datasets "https://doi.org/10.15468/9pzhm6" and "https://doi.org/10.15468/9pzhm6" belongs to one study.
Enregistrements de données
Les données de cette ressource données d'échantillonnage ont été publiées sous forme d'une Archive Darwin Core (Darwin Core Archive ou DwC-A), le format standard pour partager des données de biodiversité en tant qu'ensemble d'un ou plusieurs tableurs de données. Le tableur de données du cœur de standard (core) contient 141 enregistrements.
2 tableurs de données d'extension existent également. Un enregistrement d'extension fournit des informations supplémentaires sur un enregistrement du cœur de standard (core). Le nombre d'enregistrements dans chaque tableur de données d'extension est illustré ci-dessous.
Cet IPT archive les données et sert donc de dépôt de données. Les données et métadonnées de la ressource sont disponibles pour téléchargement dans la section téléchargements. Le tableau des versions liste les autres versions de chaque ressource rendues disponibles de façon publique et permet de tracer les modifications apportées à la ressource au fil du temps.
Versions
Le tableau ci-dessous n'affiche que les versions publiées de la ressource accessibles publiquement.
Droits
Les chercheurs doivent respecter la déclaration de droits suivante:
L’éditeur et détenteur des droits de cette ressource est Leibniz Institute for the Analysis of Biodiversity Change (LIB). Ce travail est sous licence Creative Commons Attribution (CC-BY) 4.0.
Enregistrement GBIF
Cette ressource a été enregistrée sur le portail GBIF, et possède l'UUID GBIF suivante : 3fe20fcc-d684-44e6-823a-9121d06be1ac. Leibniz Institute for the Analysis of Biodiversity Change (LIB) publie cette ressource, et est enregistré dans le GBIF comme éditeur de données avec l'approbation du GBIF Germany.
Mots-clé
Samplingevent; Specimen
Données externes
Les données de la ressource sont disponibles dans d'autres formats
| ASV-Registry | https://doi.org/10.20363/6wdc-yh16 UTF-8 Microsoft Excel |
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Contacts
- Créateur ●
- Personne De Contact
- Utilisateur
- Utilisateur
Couverture géographique
Near the town Reipoltskirchen, Rhineland-Palatinate, Germany
| Enveloppe géographique | Sud Ouest [-90, -180], Nord Est [90, 180] |
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Couverture taxonomique
Pas de description disponible
| Kingdom | Animalia |
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| Order | Symphypleona, Heteronemertea, Mermithida, Hemiptera, Sarcoptiformes, Opiliones, Philodinida, Sordariales, Pucciniales, Glomerellalles, Tubulaniformes, Polydesmida, Monostilifera, Enchytraeida, Adinetida, Onygenales, Siphonaptera, Ceramiales, Julida, Plectida, Raphidioptera, Blattodea, Microascales, Ixodida, Crassiclitellata, Glomerida, Thysanoptera, Neuroptera, Dermaptera, Parachaela, Anthoathecata, Erysiphales, Ephemeroptera, Araneae, Helotiales, Mucorales, Agaricales, Mesostigmata, Plecoptera, Chordeumatida, Triganglionata, Lepidoptera, Ostropales, Pleosporales, Diptera, Teloschistales, Entomobryomorpha, Glomerellales, Trichoptera, Arthoniales, Geophilomorpha, Polyporales, Neelipleona, Anura, Sporidiobolales, Lithobiomorpha, Eurotiales, Mycocaliciales, Orthoptera, Rhabditida, Lecanorales, Russulales, Hypocreales, Coleoptera, Chaetothyriales, Peltigerales, Mecoptera, Hymenoptera, Thelephorales, Boletales, Peronosporales, Psocodea, Cantharellales, Trombidiformes, Clupeiformes, Stylommatophora, Cystobasidiales, Poduromorpha, Dothideales, Isopoda |
Couverture temporelle
| Date de début / Date de fin | 2022-05-10 / 2022-08-26 |
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Données sur le projet
The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.
| Titre | DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC) |
|---|---|
| Identifiant | P142690-DNA-MB_2025 |
| Financement | Heinrich Böll foundation (P142690) and Trier University of Applied Sciences: Junior Researcher Fund |
Méthodes d'échantillonnage
The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.
| Etendue de l'étude | The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'. |
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Description des étapes de la méthode:
- The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.
Citations bibliographiques
- Hoffmann L, Stoll S, Rach B (2026). Butterfly abundance data of four different short rotation coppice (SRC) life stages. Version 1.2. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/9pzhm6 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/9pzhm6
- Hoffmann L, Stoll S, Huber J, Rach B (2026). Vegetation data of four different short rotation coppice (SRC) life stages. Version 1.6. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/phmw92 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/phmw92
Métadonnées additionnelles
| Identifiants alternatifs | 3fe20fcc-d684-44e6-823a-9121d06be1ac |
|---|---|
| https://biocase.zfmk.de/ipt/resource?r=dna_metabarcoding_data_of_arthropods_src |