DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)

Sampling event
Latest version published by Leibniz Institute for the Analysis of Biodiversity Change (LIB) on Feb 2, 2026 Leibniz Institute for the Analysis of Biodiversity Change (LIB)
Publication date:
2 February 2026
License:
CC-BY 4.0

Download the latest version of this resource data as a Darwin Core Archive (DwC-A) or the resource metadata as EML or RTF:

Data as a DwC-A file download 141 records in English (3 MB) - Update frequency: unknown
Metadata as an EML file download in English (41 KB)
Metadata as an RTF file download in English (15 KB)

Description

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'. The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

This data and the data in the datasets "https://doi.org/10.15468/9pzhm6" and "https://doi.org/10.15468/9pzhm6" belongs to one study.

Data Records

The data in this sampling event resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 141 records.

2 extension data tables also exist. An extension record supplies extra information about a core record. The number of records in each extension data table is illustrated below.

Event (core)
141
dnaDerivedData 
35766
Occurrence 
35766

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is Leibniz Institute for the Analysis of Biodiversity Change (LIB). This work is licensed under a Creative Commons Attribution (CC-BY 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 3fe20fcc-d684-44e6-823a-9121d06be1ac.  Leibniz Institute for the Analysis of Biodiversity Change (LIB) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by GBIF Germany.

Keywords

Samplingevent; Specimen

External data

The resource data is also available in other formats

ASV-Registry https://doi.org/10.20363/6wdc-yh16 UTF-8 Microsoft Excel

Contacts

Lara Hoffmann
  • Originator ●
  • Point Of Contact
Author, Content Contact, Data Owner
University of Duisburg-Essen
Duisburg
DE
Stefan Stoll
  • Originator
PI, Data Owner
University of Applied Sciences Trier
Trier
DE
Dominik Buchner
  • Originator
Author, Data Owner
University of Duisburg-Essen
DE
Birgit Rach
  • User
Data Curator
Leibniz institute for the Analysis of Biodiversity Change - LIB
Bonn
DE
Birgit Rach
  • User
Data curator
LIB - Leibniz Institute for the Analysis of Biodiversity Change
DE
University of Duisburg-Essen
Owner institute
DE
University of Applied Sciences Trier
Owner institute
DE

Geographic Coverage

Near the town Reipoltskirchen, Rhineland-Palatinate, Germany

Bounding Coordinates South West [-90, -180], North East [90, 180]

Taxonomic Coverage

No Description available

Kingdom Animalia
Order Symphypleona, Heteronemertea, Mermithida, Hemiptera, Sarcoptiformes, Opiliones, Philodinida, Sordariales, Pucciniales, Glomerellalles, Tubulaniformes, Polydesmida, Monostilifera, Enchytraeida, Adinetida, Onygenales, Siphonaptera, Ceramiales, Julida, Plectida, Raphidioptera, Blattodea, Microascales, Ixodida, Crassiclitellata, Glomerida, Thysanoptera, Neuroptera, Dermaptera, Parachaela, Anthoathecata, Erysiphales, Ephemeroptera, Araneae, Helotiales, Mucorales, Agaricales, Mesostigmata, Plecoptera, Chordeumatida, Triganglionata, Lepidoptera, Ostropales, Pleosporales, Diptera, Teloschistales, Entomobryomorpha, Glomerellales, Trichoptera, Arthoniales, Geophilomorpha, Polyporales, Neelipleona, Anura, Sporidiobolales, Lithobiomorpha, Eurotiales, Mycocaliciales, Orthoptera, Rhabditida, Lecanorales, Russulales, Hypocreales, Coleoptera, Chaetothyriales, Peltigerales, Mecoptera, Hymenoptera, Thelephorales, Boletales, Peronosporales, Psocodea, Cantharellales, Trombidiformes, Clupeiformes, Stylommatophora, Cystobasidiales, Poduromorpha, Dothideales, Isopoda

Temporal Coverage

Start Date / End Date 2022-05-10 / 2022-08-26

Project Data

The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

Title DNA metabarcoding data of arthropods collected in different life stages of short rotation coppices (SRC)
Identifier P142690-DNA-MB_2025
Funding Heinrich Böll foundation (P142690) and Trier University of Applied Sciences: Junior Researcher Fund

Sampling Methods

The arthropods were collected in four different life stages of short rotation coppices (harvested, young (2 years), mature (3 years) and old (4 years)) using 3 different trapping techniques: branch sampling (BS), coloured canopy Malaise traps (MT) and pitfall traps (PIT). In each life stage, three sets of traps were placed (3 sites per life stage) and activated for two weeks, each in May, June, July and August. Once in a month, a branch sampling was conducted. In the branch sampling, 16 trees within a radius of 20m around the canopy Malaise traps were randomly selected and shaken for 10 s. Arthropods fell on a plastic tarpaulin of 1x1 m that was emptied into a collection bottle where the arthropods were stored in 96.7% ethanol.

Study Extent The dataset comprises presence data of arthropods, but also on the groups 'Annelida', 'Bacillariophyta', 'Ascomycota', 'Basidiomycota', 'Bryozoa', 'Chordata', 'Cnidaria', 'Echinodermata', 'Glomeromycota', 'Haptophyta', 'Mollusca', 'Mucoromycota', 'Nematoda', 'Nemertea', 'Ochrophyta', 'Oomycota', 'Porifera', 'Pseudomonadota', 'Rhodophyta', 'Rotifera' and 'Tardigrada'.

Method step description:

  1. The samples were analysed using DNA metabarcoding. In DNA metabarcoding, the Cytochrome Oxidase I-Region was targeted using the primers fwhF2 (forward) and fwhR2n (reverse) from Vamos et al 2017 (https://doi.org/10.3897/mbmg.1.14625) The sequences found in the samples were matched with sequences in the BOLD database. The sequences displayed are already grouped like it is known from OTUs. For this grouping, all sequences with a similarity of 97% were compiled, which means that the grouped sequences finally comprise different genetic variants of the same taxa. For each hit in the database, a plausibility check was performed by comparing the distribution range of a species (calculated from GBIF coordinates) and the trapping locations. For each detection of a sequence in a sample, the number of reads is also given. A flagging system helps the user to estimate the degree of uncertainty arising from each species hit.

Bibliographic Citations

  1. Hoffmann L, Stoll S, Rach B (2026). Butterfly abundance data of four different short rotation coppice (SRC) life stages. Version 1.2. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/9pzhm6 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/9pzhm6
  2. Hoffmann L, Stoll S, Huber J, Rach B (2026). Vegetation data of four different short rotation coppice (SRC) life stages. Version 1.6. Leibniz Institute for the Analysis of Biodiversity Change (LIB). Occurrence dataset https://doi.org/10.15468/phmw92 accessed via GBIF.org on 2026-01-30. https://doi.org/10.15468/phmw92

Additional Metadata

Alternative Identifiers 3fe20fcc-d684-44e6-823a-9121d06be1ac
https://biocase.zfmk.de/ipt/resource?r=dna_metabarcoding_data_of_arthropods_src